The Toxic Influence of The Fungicide Remilitine and Chromium Ion on Reproduction of Earthworm, Eisnia feditia

Document Type : Original Article


1 Department of Zoology, Faculty of Science, University of Sirte, Libya

2 Department of biology, Faculty of Science, University of Ghemines, Libya


In The present study, The E.fetida has maintained in a glass aquaria on a culture media as described by Organization for Economic Cooperation and Development (OECD, 2004), at room temperature of 20±2 ºC. The food consisted of artificial soil mixed with barley grains powder as a food supplement every week throughout the test period. The moisture conditions of the rearing soil were started at approximately 60% water holding capacity. The moisture, thereafter, was maintained by regularly sprinkling water on the soil. Fungal growth was removed when observed on the soil surface. Rearing soil was changed every eight weeks until the worms required for the experiment were with an average weight of 7 to 9 grams. In this experiment, the adult worms were exposed to the artificial soil (Van EGmond, Postbus 24, 2230AA Rijnsbury) contaminated with one concentration of 50 mg/L chromium + Remilitine 500mg/L., respectively. The artificial soil used (OECD, 2004) consisted of 70% quartz sand, 20% kaolin clay, 10% sphagnum peat and calcium carbonate to adjust the pH to 5-6.5. A weight of 250 grams of soil weighted by (Electronic scale soil) was transferred into glass containers (12 cm width, 15cm length, 20 cm high) to which 100 ml of chromium + Remilitine were added and mixed thoroughly. Each treatment was replicated three times and a control site with three replicates were set using plain water. the number of cocoon production by control worms were compared with the cocoon numbers produced by the chrome-Remiltine treated worm using t-test, the results clearly showed a significant difference (F=8.47 P<0.04) after 28-day period, the mean ± S.D of cocoon produced by control worms were 46.67±2.082 compared to 37.00 ± 9.29 of chrome-Remiltine at the 28-day post treat.