Influence of Galangal Root Oil on Some Heat Shock Proteins and Antioxidant Enzymes in H2O2-Induced Hepatic Damage in Rats

Ghassan, Raya; Alchalabi, Ali S.; Shaher, Waad S.

doi:10.21608/eajbsz.2025.464859

Influence of Galangal Root Oil on Some Heat Shock Proteins and Antioxidant Enzymes in H2O2-Induced Hepatic Damage in Rats

Document Type : Original Article

Authors

¹ Department of biology, Education college for Women, University of Mosul, Mosul, Iraq.

² Department of clinical laboratory science, College of Pharmacy, University of Mosul, Mosul, Iraq.

³ Department of biology, College of Science, University of Mosul, Mosul, Iraq.

10.21608/eajbsz.2025.464859

Abstract

The study examined how Galangal root oil affected heat shock proteins (HSPs) and antioxidant enzymes in rats with H₂O₂-induced liver injury. 24 adult male rats 200-230g BW were randomly assigned to four groups: the control group received 0.1 N.S. orally, and three treatment groups: Group 1 (G1) had liver damage from daily 2.5% H₂O₂in drinking water, with water replaced twice daily after two weeks of acclimation. Group 2 (G2) got 0.2 ml of 100% Pure Therapeutic Grade Galangal Root Oil (GRO) orally each rat. Group 3 (G3) received 0.2 ml GRO/ H₂O₂. The trial lasted 30 days. Molecular analysis was performed on liver samples after the trial. H₂O₂increased HSPs and decreased antioxidant enzyme expression throughout the research. This impact is enhanced by GRO alone/with H₂O₂. It was found that 2.5% H₂O₂exposure has serious biological effects, while galangal root oil protects hepatocytes from severe oxidative stress via modifying heat shock protein and antioxidant enzyme regulatory genes.

Keywords