Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines

Document Type : Original Article

Authors

1 Department of zoology faculty of science Ain shams university,Cairo.Egypt.

2 Biomedical informatics and chemo informatics group, informatics and systems department, National Research Center, Dokki, Giza, Egypt.

3 Hormones Department, Medical Research and Clinical Studies Institute, and Stem Cell lab, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, Giza, Egypt.

Abstract

The role of miR -98 is controversial in the literature which we aimed to clarify in breast cancer cell lines.  To elucidate the role of miR-98, mimic and inhibitor were transfected into MCF-7and MDA-MB-231 cell lines. Q-RT-PCR was used to analyze Wnt pathway gene expression. Function analysis including wound healing, and determining oxidative stress parameters were conducted. miR-98 was up-regulated in most breast cancer tissue databases. Bioinformatics analysis target prediction and enrichment analysis clarified that miR-98 is incorporated in oxidative stress pathway. It targets several genes from which HIF-1A and VEGFA which were selected for real time analysis. Q-RT- PCR results revealed that HIF-1A and VEGFA were non-significantly increased upon inhibition of miR-98 in MCF-7 while HIF-1A and VEGFA were significantly decreased upon inhibition of miR-98 in MDA-MB-231. On the other hand, the level of catalase which is ROS tracker enzyme in cell lysate of MCF-7 was significantly increased upon inhibition of miR-98. miR-98 triggered the cells of MCF-7 and MDA-MB-231 to acquire great proliferative and migrative power assisting it to heal the manufactured scratch in both cell lines as compared to control. Overall, our result point out that miR-98 acts as oncomiR in breast cancer cell lines.

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