Ameliorative Effect of Vitamin C against Hydroxyurea-Induced Hepatic Toxicity in Male Rats

Document Type : Original Article

Authors

Department of Zoology, Faculty of Sciences, University of Sirte, Sirte, Libya.

Abstract

Background and aim: Hydroxyurea (HU) possesses several characteristics of an ideal drug for various diseases, despite the reported side effects. The current study intends to search for the potential ameliorative effectiveness of vitamin C on hydroxyurea-induced hepatotoxicity in a rat model. Materials and methods: 24 adult male albino rats were split into four groups. Group I served as the control; Group II received 5 mg/kg bw of vitamin C; Group III received 100 mg/kg bw of HU; and Group IV received a combination of 5 mg/kg bw VC and 100 mg/kg bw HU, which were administered orally for 30 days to assess liver function tests, oxidative stress, histopathology, and the detection of DNA damage. Results: The results demonstrated a significant decline (p < 0.01) in the absolute organ weight of rats treated with HU. However, there was no significant contrast in the liver-relative condition. The rats exhibited elevated levels of AST, ALT, and MDA a reduction in albumin, total protein, SOD, CAT, and GSH. The group that received HU displayed signs of degeneration, hydropic deterioration, and fatty degeneration. A comet assay revealed an increase in tail length and DNA content in liver cells. There was no significant difference in olive tail moment but a significant decrease in % tailed, % DNA in the tail, and tail moment. Conclusion: The administration of VC with HU improved liver function tests, oxidative stress, and histopathology compared to the HU group.

Keywords

Egyptian Academic Journal of Biological Sciences, B. Zoology
Volume 16, Issue 2
December 2024
Pages 39-50

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