Anti-Tumor Effect of Cicer Arietinum against the Triple-Negative Breast Cancer Cells

Hassan, Hebatallah; Sayed, Amany Ahmed

doi:10.21608/eajbsz.2019.36226

Anti-Tumor Effect of Cicer Arietinum against the Triple-Negative Breast Cancer Cells

Document Type : Original Article

Authors

1-Department of Zoology, Faculty of Science, Cairo University, Giza, 12613, Egypt. 2- Structural Biology Laboratory, Egypt Nanotechnology Center (EGNC), Cairo University, El-Sheikh Zayed Campus, Zayed City, Giza, Egypt.

10.21608/eajbsz.2019.36226

Abstract

Triple negative breast cancer (TNBC) is the most aggressive and deadly subtype of breast cancer. The only treatment option available for TNBC is conventional therapy, which associated with debilitating-side effects and drug-resistance. Therefore, using natural chemo-preventive agents to suppress cancer progression or reverse its initiation is an effective and safe way in cancer therapy. The aim of this study was to investigate the antitumor effect of Cicer arietinum (CAE) in vitro employing TNBC cell lines MDA-MB-231 and 4T1. Cell viability, Colony formation as readout for single cell ability to form a colony, gelatin-cell adhesion, and immunofluorescence staining were used to assessthat effect. Relative to control cells, our results indicate that CAE treatment significantly decreased cell viability, colony formation, and cell adhesion. Intriguingly, immunofluorescence microscopy unveiled that TNBC cells exhibited an attenuation of the constitutive and EGF-induced p44/42 MAPK signaling pathway implicated in regulating cell survival. Moreover, a morphological transition of TNBC cells from a fibroblast-like spindle-shaped into epithelial-like cells was observed. In conclusion, CAE may emerge as a promising antitumor agent against TNBC possibly via attenuation of EGF-induced p44/42 MAPK signaling.

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