Comparative Acute Exposure Study of Abamectin Different Formulations Inducing Physiological and Oxidative Stress Biomarkers in Nile Tilapia, (Oreochromis niloticus).

Document Type : Original Article


1 Plant Protection Dept., Faculty of Agriculture, Zagazig University, P.O. Box, 44511, Zagazig, Egypt.

2 Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, P.O. Box 44511, Zagazig, Egypt.


The goal of this study is to assess the acute and sublethal potential hazards on tilapia fish (Oreochromis niloticus) exposed to two different commercial formulations of abamectin (ABM, 5%): emulsifiable concentration (EC) and microemulsions (ME). The median lethal concentration (LC50 - 96 h) to each formulation was determined, as well as the adverse effects of sublethal concentrations (½ LC50) on physiological and oxidative stress biomarkers after 96hs of exposure. The LC50 (96 h) values for EC and ME were 10 and 16.6 µg/L, respectively. Furthermore, in both EC and ME, the findings of physiological and oxidative stress aspects revealed significant markedly increases in serum total protein, albumin, globulin, and urea, as well as a significant decline in aminotransferases activity (ALT and AST), and creatinine levels (Cre). Moreover, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione peroxidase (GPx) were greatly increased, whereas glutathione-S-transferase (GST) and glutathione (GSH) were significantly reduced in the liver and brain.