Possible Ameliorative Role of Ethanolic Ginger Extract (Zingiber officinale) against Testicular Toxicity Induced By Monosodium Glutamate In Albino Rats

Document Type : Original Article


Zoology Department. Faculty of Women for Arts, Science and Education, Ain Shams University. Cairo, Egypt


The paper aims to investigate the protective effect of ethanolic ginger extract against Monosodium glutamate (MSG) induced reproductive disorders in male rats. Forty-eight male albino rats were separated into four experimental groups:  normal control, ginger extract, MSG only, and MSG plus ginger extract groups. All experimental regimens were administrated orally for four weeks. Dissection was done after two and four weeks from the starting of the experiment. Body and testis weight, sperm abnormalities, biochemical investigations were recorded. Additionally, molecular, histopathological, and immunohistochemical techniques in testis tissue were performed. The present data shows a significant increase in body weight, sperm abnormalities, and serum total cholesterol, with a significant decline in testis weight, serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), and testosterone levels after MSG administration. There was an upregulation in the expression of Bax protein whereas downregulation in the expression of B-cell lymphoma-2 (BCL-2) protein in the MSG group when compared with the normal control. Furthermore, testicular alterations were distinguished in the group treated with MSG only, along with enhanced caspase-3 and declined BCl-2 positive cells in testis tissue. While administration with ethanolic ginger extract showed an ameliorative effect contrasted with testicular alterations produced by Monosodium glutamate.