Exposure to Lufenuron During the Third Gestational Period Induces Genotoxicity and Oxidative Stress Effects in Pregnant Albino Rats and Their Fetuses

Document Type : Original Article


1 Department of Zoology, Faculty of Science, Cairo University, Egypt

2 Department of Zoology, Faculty of Science, Cairo University, Egypt.


The last few decades witnessed massive increments in the accumulation of agrochemicals and insecticides residues in the environment, poisoning human and animal food. In our study, we evaluated the genotoxic, and oxidative stress effects of residual-level lufenuron exposure on pregnant rats during the third period of gestation (day 13-19) to both mother and fetuses. The experimental design depended on dividing the females into three groups; control (untreated), low-dose group (orally administered with 0.4 mg/kg lufenuron) and high-dose group (orally administered with 0.8 mg/kg lufenuron). In comet assay, the liver cells of lufenuron-treated pregnant dams and their fetuses showed significant DNA damage compared to control.  Cell cycle arrest was also detected in liver cells at G0/G1 phase through flow cytometry. The oxidative stress induced by lufenuron in liver cells of mothers and fetuses was detected by the increased lipid peroxidation as indicated by the elevated malondialdehyde (MDA) levels- and decreased levels of enzymatic antioxidants (glutathione peroxidase [GPx] and superoxide dismutase [SOD]). In conclusion, the obtained results point out the high potential of chronic exposure to lufenuron residual concentrations during the third period of gestation for exerting genotoxic, and oxidative stresses on pregnant rats and their fetuses.