Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Aref, Abdel-Baset M.; Ünlü, Gülhan; Ahmed, Osama M.; Ali, Lobna A.; Semmler, Margit

doi:10.21608/eajbsz.2021.177492

Possible Short-Term Biological Effects of Kefir: Ⅱ: Protective Role and Therapeutic Efficacy of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Liver of High-Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Document Type : Original Article

Authors

¹ -Cell Biology and Histochemistry Division, Zoology Department, Faculty of Science, South Valley University, Qena, Egypt. -Vice President of Institutional Animal Cure and Use Committee of South Valley University in Egypt (IACUC-SVU-Egypt).

² Department of Animal, Veterinary, and Food Sciences, University of Idaho, USA.

³ Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Salah Salem Street, P.O. Box 62514, Beni-Suef, Egypt.

⁴ Cell Biology and Histochemistry Division, Zoology Department, Faculty of Science, South Valley University, Qena, Egypt.

⁵ Diabetes Research Institute, Düsseldorf University, Düsseldorf, Germany

10.21608/eajbsz.2021.177492

Abstract

This study was designed to investigate the protective role of kefir and insulin consumption on cellular activities, histochemical components, histological architecture, and integrity of the liver by measuring IL-6 and TNF-α in high fat-fed STZ- induced diabetic male Wistar rats.
            From a cell biological and histochemical point of view, both kefir and insulin have beneficial various effects on cellular activities and the histochemical components such as DNA, RNA, total protein, collagen, polysaccharides, and phospholipids materials synthesis in the examined normal and diabetic hepatocytes of the male rats. Biochemically, kefir and insulin have various biochemical effects on the serum IL-6 and TNF-α levels.
           From a pathological point of view, we can say the beneficial various cell biological and histochemical effects of kefir, pathologically may make kefir, to some extent, completely repair all the pathological side effects of type1 diabetes on the hepatocytes in diabetic rats. Also, insulin repair some of the pathological side effects of diabetes on the hepatocytes.
           The positive results of using kefir and insulin in treating the pathogen effects of diabetes make it possible to obtain positive clinical applications/implications. Therefore, these positive results encourage us to continue working and complete the various long-term pre-clinical trials and clinical trials phases.

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